The Korean Journal of Pharmacology (대한약리학회지)
- Volume 27 Issue 2
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- Pages.145-153
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- 1991
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- 0377-9459(pISSN)
Human Neutrophil Cathepsin G: In Vivo Synthesis of Anti-HNCG Antibody, Inhibition of the Activity of HNCGs and Mechanism of the Inhibitions
사람 호중구 Cathepsin G: Anti-HNCG Ab의 In Vivo 합성, HNCG의 활성도 억제와 그 기전에 관한 연구
- Bae, Sung-Jun (Department of Pharmacology, Kosin Medical College) ;
- Kim, Woo-Mi (Department of Pharmacology, Kosin Medical College) ;
- Kim, Ki-Chan (Department of Pharmacology, Kosin Medical College) ;
- Chang, Myung-Woong (Department of Microbiology, Kosin Medical College) ;
- Kang, Koo-Il (Department of Pharmacology, Kosin Medical College)
- 배성준 (고신대학 의학부 약리학교실) ;
- 김우미 (고신대학 의학부 약리학교실) ;
- 김기찬 (고신대학 의학부 약리학교실) ;
- 장명웅 (고신대학 의학부 미생물학교실) ;
- 강구일 (고신대학 의학부 약리학교실)
- Published : 1991.12.30
Abstract
Human neutrophil cathepsin-G, which has been known as one of the active enzymes causing inflammatory diseases, was purified by two steps procedure involving one size exclusion (Ultorogel AcA54) and one ion exchange (CM-Sephadex) chromatography. Purified HNCGs were cross-reacted with Anti-HNCathepsin-G antibodies which were radised in rabbits and purified by cathepsin-G labeled Sepharose 4B affinity chromatography. HNCGs were effectively inhibited by NSAIDs including phenylbutazone, sulindac, oxyphenbutazone, salicylic acid and salicyluric acid.
염증성 질환의 원인 인자중 하나로 알려진 사람 호중구 Cathepsin G를 두단계의 크로마토그라피를 거쳐 분리하였다. 이 순수 분리된 효소를 이용하여 토끼에서 항체를 In Vivo 합성하고 그 혈액으로부터 순수 항체를 분리하였다. NSAIDs 약제중 phenylbutazone, sulindac, oxyphenbutazone, salicilic acid등은 이 효소를 강력하게 억제하였으며