Spectrophotometric Determination of Amantadine Sulfate after Ion-Pairing with Methyl Orange

  • Choi, Kyong (College of Pharmacy, Chonnman National University) ;
  • Choi, Jung-Kap (College of Pharmacy, Chonnman National University) ;
  • Yoo, Gyurng-Soo (College of Pharmacy, Chonnman National University)
  • Published : 1991.12.01

Abstract

A convenient spectrophotometric method was examined for the determination of amantadine sulfate (AMTS) which has no UV-VIS chromopohores. AMTS was ion-paired quantitatively with methyl orange (MO) at $70^{\circ}C$ for 30 min. The ion-paired complex was extracted with dichloromethane and the absorbance was measured at 421.5 nm. A linear relationship was observed in the range of $2.5{\times}10^{-7}\;M$ to $3.75{\times}10^{-6}\;M$ and the correlation coefficient was 0.999 (n=3). This assay method was applied to the quantification of AMTS in commercial tablet form with good recovery and high precision.

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