Purification of an Xylanase from the Extracellular Xylanolytic Systems of Trichoderma viride and Hydrolysis of Xylan

Trichoderma viride 균체외 효소로 부터 Xylanase의 정제 및 Xylan의 분해

  • Eom, Tae-Jin (College of Agriculture, Kyungpook National University)
  • Received : 1991.02.07
  • Published : 1991.06.30

Abstract

The endo-1,4-${\beta}$-xylanase was extracted and purified from the extracellular xylanolytic systems of Trichoderma viride. The crude enzyme was chromatographed with ion-exchange reins of DEAE Sepharose CL-6B, Sepharose, S-Sepharose CL-6B and the resulting xylanase was turned out to be a single protein as 20KD hy SDS-polyacrylamide gel electrophoresis. The xylooligomers were obtained from xylan by incubation with the purified xylanase up to 50%. The ${\beta}$-xylosidase lost its activity completely by incubation of crude enzyme for 24hr with buffer solution of pH 2.8 at $27^{\circ}C$. And also, the xylooligomers were obtained from xylan as a main product by incubation with the crude enzyme treated with acidic buffer.

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