Studies on the Enzymatic Hydrolysis of Lignocellulosic Materials for the Alternative Fuels(III) - Quantitative Recycling of Cellulase Enzyme in the Enzymatic Hydrolysis of Steam-Exploded Woods -

대체연료(代替燃料) 생산(生産)을 위한 목질재료(木質材料)의 가수분해(加水分解)에 관한 연구(硏究) (III) - 폭쇄(爆碎)처리재의 산소분해시(酸素分解時) Cellulase 산소(酸素)의 정량적(定量的) 회수(回收)에 관하여 -

Steam-exploded woods were delignified by two-stage with a 0.3% NaOH extraction and oxygen-alkali bleaching and were subjected to the enzymatic hydrolysis with cellulase enzyme. Also, an improved almost quantitative recycle process of cellulase enzyme was discussed. In enzyme recovery by affinity method, The first recycling showed relatively high hydrolysis rate of 96.4%. Even at the third recycle, hydrolysis rate was 87.0 percents. In the case of cellulase recovery by ultrafiltration method, first 2 recycling treatments resulted in very high hydrolysis rates, 96.8% and 95.0%, respectively. Even the third recycling showed about 93.6%. Steam-explosion treatment of oak wood followed by 2-stage delignification with alkali and oxygen-alkali produced a excellant substrate for the enzymatic hydrolysis that allowed almost quantitative recycle of cellulase.