Study on Pregnancy and Improvement of Fertilization rate by Micromanipulation (Partial Zona Dissection (PZD) and Micro-Insemination by Sperm Transfer(MIST) in human IVF-ET

체외수정시술시 미세조작술에 의한 수정률 향상과 임신에 관한 연구

  • Lee, Ho-Joon (IVF Research Laboratory, Department of Obstetrics and Gynecology, Cheil General Hospital) ;
  • Choi, Kyoo-Wan (IVF Research Laboratory, Department of Obstetrics and Gynecology, Cheil General Hospital) ;
  • Jun, Jong-Yung (IVF Research Laboratory, Department of Obstetrics and Gynecology, Cheil General Hospital) ;
  • Park, Jong-Min (IVF Research Laboratory, Department of Obstetrics and Gynecology, Cheil General Hospital) ;
  • Kwon, Hyuck-Chan (IVF Research Laboratory, Department of Obstetrics and Gynecology, Cheil General Hospital) ;
  • Kim, Moon-Kyoo (Department of Biology, College of Natural Science, Hanyang University)
  • 이호준 (제일병원 산부인과 체외수정 연구실) ;
  • 최규완 (제일병원 산부인과 체외수정 연구실) ;
  • 전종영 (제일병원 산부인과 체외수정 연구실) ;
  • 박종민 (제일병원 산부인과 체외수정 연구실) ;
  • 권혁찬 (제일병원 산부인과 체외수정 연구실) ;
  • 김문규 (한양대학교 자연과학대학 생물학과)
  • Published : 1990.06.30

Abstract

The purpose of this study is to improve fertilization rate in IVF-ET program of patients with male infertility used micromanipulation technique, partial zona dissection (PZD) or micro-insemination by sperm transfer (MIST). The results were as follows 1. The fertilization rate of non-micromanipulated oocytes and micromanipulated (PZD) oocytes were 12.5% (n=2) and 42.2% (n=19), respectively, and showed significant differences between two groups (p<0.05). 2. The fertilization rate of micromanipulated (MIST) oocytes was 30% (n=27). 3. The damage rate of Group 1 (PZD) and Group 2 (MIST) were 15.7% (3/19) and 29.6% (8/27), respectively. 4. One pregnancy resulted following replacement of micromanipulated (MIST) embryos in 4 patients.

Keywords