Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Partial Purification of Antifertilizing Factor from Seminal Plasma

정장내의 Antifertilizing factor의 분리 및 정제

  • Kim, S.W. (Infertility Clinic, Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University) ;
  • Baik, C.S. (Infertility Clinic, Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University) ;
  • Kim, J.M. (Infertility Clinic, Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University) ;
  • Suh, B.H. (Infertility Clinic, Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University) ;
  • Lee, J.H. (Infertility Clinic, Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University)
  • 김수원 (경희대학교 의과대학 산부인과학교실 불임 크리닉) ;
  • 백청순 (경희대학교 의과대학 산부인과학교실 불임 크리닉) ;
  • 김재명 (경희대학교 의과대학 산부인과학교실 불임 크리닉) ;
  • 서병희 (경희대학교 의과대학 산부인과학교실 불임 크리닉) ;
  • 이재현 (경희대학교 의과대학 산부인과학교실 불임 크리닉)
  • Published : 1990.11.30
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Abstract

Early studies demonstrated that seminal plasma has a factor which inhibits fertilizing ability in a reversible manner. The factor can be precipitated by centrifugation at 104000g for 18 hr. The precipitate was applied to a CM cellulose column and eluted with high salt concentration. This fraction possessed antifertilizing activity was applied to a Sephacryl S-200 column according to a modification of the method of Reddy et al. Using such inhibition of in vitro fertilization ability as an assay, we have carried our experiments to purified the factor. When the factor was added to IVF medium, 70-80% of fertilization was inhibited.

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