AUTORADIOGRAPHIC STUDY OF THE COLLAGENASE - INFLUENCE ON THE RAT PERIODONTIUM AFTER EXPERIMENTAL TOOTH MOVEMENT

실험적(實驗的) 치아이동후(齒牙移動後) 교원효소(膠原酵素) 투여(投與)가 치근막(齒根膜) 섬유(纖維)의 변화(變化)에 미치는 영향(影響)에 관(關)한 자기방사법적(自己放射法的) 연구(硏究)

The purpose of this study was to analyze the reorganization of periodontal ligament after collagenase treatment with autoradiography. The author compared the collagenase-treated experimental group and no-treated experimental group with control group. Fourty eight Sprague-Dawley rats were divided into nine groups, including normal control and immediate group. Closed coil springs were used between the upper incisors and the first molars with 100 grams. Collagenase and $^3H-proline$ were adminstered and the samples were sacrificed and sectioned. After being dipped into the NTB-3 emulsion the samples were analyzed with light microscope under H/E stain. Data were analyzed by t-test and ANOVA. The results were as follows: 1) Generally collagenase-treated groups got more $^3H-proline$ uptake than no-treated groups. 2) Compared with normal control group, collagenase-treated group had the same $^3H-proline$ uptake in amount at 21th day. 3) Among cemento-enamel junction, middle, apex areas, cementa-enamel junction area of collagenase-treated group arrived at normal control level earlier than no-treated group. 4) Cemento-enamel junction area had the most $^3H-proline$ incorporation amount in no-treated group, but apex area had the most in collagenase group.