Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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A STUDY ON THE CYTOTOXICITY OF CYTOSINE ARABINOSIDE AND VINBLASTINE ON CULTURED MOUSE FIBROBLASTS

섬유모세포에 미치는 세포 독성에 관한 연구

  • Kim, Jae-Min (Department of Ocular Optics, Dongshin Junior College) ;
  • Kim, Ki-Won (Department of Biology, College of Natural Science, Chonnam National University) ;
  • Chung, Yeun-Tai (Department of Anatomy, School ofMedicine, Wonkwang University)
  • 김재민 (동신전문대학 안경광학과) ;
  • 김기원 (전남대학교 자연과학대학 생물학과) ;
  • 정연태 (원광대학교 의과대학 해부학교실)
  • Published : 1990.06.01
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Abstract

Cytotoxic effects of cytosine arabinoside and vinblastine on cultured fibroblasts were determined by colorimetric assays of neutral red (NR) and tetrazolium MTT, and by mutagenicity tests . Cytosine arabinoside and vinblastine were highly toxic by showing that concentrations of NR-50 and MTT-50 of two drugs were lower than 100 ${\mu}$M. At mid-point cytotoxicityvalue of two drugs, frequencies of micronuclei and SCEs were very high and chromosome showed structural abnormalities. The sizes of micronuclei formed by vinblastine were larger than those induced by cytosine arabinoside. These results suggest that cytosine arabinoside and vinblastine have highy mutagenic and severe cytotoxic effects on the cultured mouse fibroblasts.

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