The Korean Journal of Nuclear Medicine (대한핵의학회지)

The Korean Society of Nuclear Medicine (대한핵의학회)
권호 표지

Distribution of Radionuclide Labeled Liposome in Experimental Study

방사성동위원소표지 Liposome의 분포에 대한 실험적 연구

  • Lee, Bum-Woo (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Jeong, Jae-Min (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Kim, Sang-Eun (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Lee, Dong-Soo (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Chung, June-Key (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Lee, Myung-Chul (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Koh, Chang-Soon (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Ha, Sung-Whan (Department of Therapeutic Radiology, College of Medicine, Seoul National University)
  • 이범우 (서울대학교 의과대학 내과학교실) ;
  • 정재민 (서울대학교 의과대학 내과학교실) ;
  • 김상은 (서울대학교 의과대학 내과학교실) ;
  • 이동수 (서울대학교 의과대학 내과학교실) ;
  • 정준기 (서울대학교 의과대학 내과학교실) ;
  • 이명철 (서울대학교 의과대학 내과학교실) ;
  • 고창순 (서울대학교 의과대학 내과학교실) ;
  • 하성환 (서울대학교 의과대학 치료방사선과학교실)
  • Published : 1990.11.25
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Abstract

Liposome was labelled with $^{99m}Tc$ after negative charged liposome was formed with combination of a few lipid components. $^{99m}Tc$ liposome was injected through the tail vein of C3H mice bearing fibrosarcoma and biodistribution of $^{99m}Tc$ liposome was evaluated. The results were as follows: 1) We confirmed formation of liposome which was small unillamellar and multilamellar vesicles. 2) In this experiment the optimal concentration of $SnCl_2$ was $156{\mu}g/ml$ to label liposome with $^{99m}Tc$ and labelling efficiency was 95%. 3) The labelled liposome was stable when it was incubated with human serum for 24 hours. Mean labelling efficiency was 94% at 24 hour. 4) The main uptake sites of Tc-99m liposome were liver and spleen. It showed significantly higher uptake than $^{99m}Tc$ HSA (p < 0.001). 5) $^{99m}Tc$ liposome uptake in tumor tissue was not significantly higher than $^{99m}Tc$ HSA uptake. In conclusion, $^{99m}Tc$ liposome disclosed high labelling efficiency and was highly stable. Liver and spleen were main uptake sites of $^{99m}Tc$ liposome. The uptake mechanism of $^{99m}Tc$ liposome also seemed to be different from that of $^{99m}Tc$ HSA. We conclude that $^{99m}Tc$ liposome would be a promising agents for the imaging of some tumor.

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