Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Proteolysis of Glucagon Bound to Dimyristoylphosphatidylcholine Vesicle

  • Yi, Gwan-Su (Department of Biological Science and Engineering, The Korea Advanced Institute of Science and Technology) ;
  • Kim, Hyoung-Man (Department of Biological Science and Engineering, The Korea Advanced Institute of Science and Technology)
  • Published : 1990.12.20
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Abstract

Glucagon was found to interact with DMPC vesicles electrostatically and hydrophobically. It appears that glucagon bound irreversibly to the vesicles through hydrophobic interaction was partially protected from the proteolysis by trypsin. Out of three possible sites, only the peptide bond preceded by Arg-18 was cleaved by a prolonged trypsin treatment. ${\alpha}$-chymotrysin did not affect the vesicle-bound glucagon. Based on these observations, possible structure of irreversibly bound glucagon on the vesicle surface is discussed.

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