Studies on the Induction of Transformation and Multiplication in Orchid Plants I. Formation of Somatic Embryos and Regeneration from Immature Seeds of Bletilla striata

난과식물의 형질전환 유도 및 다량증식에 관한 연구 I. 자란 (Bletilla striata)의 미성숙 종자로부터 체세포배 형성 및 식물체 재분화

Our study was carried out for plant regeneration via somatic embryogenesis from immature seeds of Bletilla striata. The highest frequency of embryogenic callus formation was obtained from the immature seeds (at 150 days after pollination) cultured on Hyponex and VW medium supplemented with 3 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l kinetin under the dark condition. Multiple somatic embryos were induced when embryogenic callus was transferred to VW medium without growth regulators under continued illumination. Somatic embryos were observed histologically with scanning electron microscopy. Regeneration of Bletilla striata was obtained from somatic embryos with a well-defined scutellum and coleoptile as well as with one or more shoot primordia and root primordia. We think that these methods for orchid multiplication must be useful to access clonal propagation of orchids.