Effect of Carbon Sources on the Synthesis of Phospholipid and Fatty Acid Composition in Chloroplast of Chlorella ellipsoidea

Chlorella ellipsoidea 엽록체의 인지질 생합성 및 지방산 조성에 미치는 탄소원의 효과

Chlorella cells were cultured with M4N media treated with glucose (5 mM) sucrose (10 mM) and raffinose (30 mM). Phospholipids and their fatty acid compositions were analyzed in the chloroplast isolated from cultured Chlorella cells. Growth rate was prominently raised in the treatment with raffinose. Glucose was the most excellent carbon source in the biosynthesis of total lipid, phosphatidylcholine(PC), phosphatidylethanolamine(PE), phosphatidylinositol(PI) of the chloroplast. Also, the major fatty acids were palmitic, linoleic and linolenic acid during the biosynthesis of phospholipid in the control and in the treatment with carbon sources.