Abstract
Bacillus sphaericus ts-Dl290 was characterized by SDS-PAGE produced by the mutant at $30^{\circ}C$ and $42^{\circ}C$. The total amount of proteins produced by the mutant at $42^{\circ}C$ decreased to one-fifth of those at $30^{\circ}C$; however, when the culture was shifted down from $42^{\circ}C$ after 4 to $30^{\circ}C$, the total amount of protein decreased to one-third and the 221 kd protein did not appear, but the 155 kd appeared remarkably. When the mutant and the wild type strain were cultured in the media containing 80$\mu g$ per ml of chloramphenicol at $42^{\circ}C$, the wild type strain synthesized half amounts of the total proteins than those at $30^{\circ}C$, and the mutant produced one-tenth of the total protein amounts. When the both strains were cultured in the media containing chloramphenicol, the 155 kd protein was produced was produced in lesser amounts than those without chloramphenicol. The 150 kd protein showed lethal activity to Culex pipiens 3rd instar larvae.
Bacillus sphaericus ts-D1290 치사돌연변이주의 특성을 연구하기 위하여 제한온도와 허용온도에서 생산하는 단백질의 차이점을 연구하였고, 모기유충에서 생산하는 단백질을 분리하여 모기유충에 치사되는지를 조사하였다. ts-D1290 균주를 $42^{\circ}C$에서 배양했을 때는 $30^{\circ}C$ 배양보다 균체의 단백질 함량이 약 1/5로 감소하였고, $42^{\circ}C$에서 4시간 배양후 $30^{\circ}C$로 배양하였들 때는 약 1/3로 감소하였다. 그리고 단백질 패천은 ts-D1290 균주를 $42^{\circ}C$에서 전배양 후 $30^{\circ}C$로 하강 배양하였을 때는 $30^{\circ}C$에서 전배야하여 $42^{\circ}C$로 상승배양하였을 때와는 달라 분자량 221kd 단백질이 나타나지 않았지만 분자량 150kd 단백질은 $30^{\circ}C$ 전 배양하였을 때와 같이 현저하게 나타났다.
