Effect of Cellobiose Octaacetate, Avicel, and KC-flock on Production of Avicelases from Penicilliurn verruculosum

Penicillium verruculosum의 Acicelase 생성에 대한 Cellobiose Octaactate와 Avicel 및 KC-flock 의 영향

During the cultivation of Penkillium uerrmulosum in the media containing cellobiose octaacetate (COA), avicel, or KC-flock as an inducer and as a sole carbon source for 21 days, cellulolytic activity and SDS-PAGE pattern of proteins in the culture broth were investigated. Protein concentration and cellulolytic activity were highest in the COA medium. As cultivation period was increased, protein content and avicel hydrolytic activity of culture broth were increased as similar extent but neither $\beta$-glucosidase nor CMC hydrolytic activity was correlated to protein content. When crude proteins from the culture broth were separated on DEAE column by HPLC, distribution of avicel-hydrolytic activities were well correlated with that of major proteins. From those results it was suggested that three major proteins having 60 K, 68 K, and 76 K of Mr. were avicel-hydrolytic enzymes.

Penicillium verruculosum 섬유소 분해효소의 유도물질인 KC-flock 및 cellobiose octaacetate(COA)를 유일한 탄소원으로 포함하는 배지에서 21일간 배양하면서 배양여액 중의 섬유소 분해효소 각 성분의 활성도와 전기영동상의 단백질 양상을 검토하였다. 배양여액의 중의 총단백질 생성 및 섬유소 분해활성의 유도 효과는 COA 배지가 다른 두 배지의 3배 이상이었다. 배양기간의 증가에 따라 배양여액 중의 총단백질량이 증가되었는데 CMC 분해활성이나 $\beta$-glucosidase 활성의 증가보다는 avicel 분해활성도의 증가가 총단백질량의 증가와 유사하다.