Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 18 Issue 3
- /
- Pages.244-250
- /
- 1990
- /
- 1598-642X(pISSN)
- /
- 2234-7305(eISSN)
Production and Characterization of Raw Starch Hydrolyzing Enzyme from Bacteria
세균에 의한 생전분 분해효소의 생성 및 특성
- Park, In-Shik (Department of Food Science and Nutrition, College of Natural Science, Dong-A University) ;
- Nam, In (Department of Food Science and Nutrition, College of Natural Science, Dong-A University) ;
- Kho, Sun-Ok (Department of Food Science and Nutrition, College of Natural Science, Dong-A University) ;
- Kim, Gi-Nahm (Department of Food Science and Nutrition, College of Natural Science, Dong-A University) ;
- Suh, Kyung-Soon (Department of Food Science and Nutrition, College of Natural Science, Dong-A University)
- 박인식 (동아대학교 식품영양학과) ;
- 남인 (동아대학교 식품영양학과) ;
- 고선옥 (동아대학교 식품영양학과) ;
- 김기남 (동아대학교 식품영양학과) ;
- 서경순 (동아대학교 식품영양학과)
- Published : 1990.06.01
Abstract
A bacterium capable of hydrotyzing raw starch was isolated from soil, which was identified as a strain of Bacillue. The effects of culture conditions and medium compositions on the enzyme production were investigated. Among tested carbon sources, soluble starch and wheat starch were most effective for the production of the enzyme, and the level of concentration for the optimal enzyme production was 0.5%. For nitrogen sources, polypeptone was best for the enzyme production, with the level of 0.5%. The enzyme was maximally produced by cultivating the organism at medium of initial pH 6.5, and temperature of
토양으로부터 생전분을 분해하는 세균을 분리하였으며, Bacillus sp.로 동정하였다. 분리한 세균으로부터 생전분 분해효소의 생성을 위한 최적조건을 검토하였다. 세균은 탄소원으로 wheat 및 soluble starch를, 질소원으로 polypeptone을 사용했을 때 최대 효소생성을 얻을 수 있었다. 그리고 탄소원을 0.5, 질소원을 0.5 수준으로 사용했을 때 효소 생성을 극대화 하였다. 그리고 배지의 초기 pH를 6.5, 배양온도를