Journal of Ginseng Research
- 제14권2호
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- Pages.207-209
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- 1990
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- 1226-8453(pISSN)
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- 2093-4947(eISSN)
인삼펩티드 유전자의 합성 및 클로닝
Chemical Synthesis and Cloning of Panax ginseng Peptide Gene
- Zhang, Hong-Ying (Enzyme Engineerin Lab, Jilin University) ;
- Chen, Dong-Song (Enzyme Engineerin Lab, Jilin University) ;
- Zhang, Jin (Enzyme Engineerin Lab, Jilin University)
- 발행 : 1990.08.01
초록
The sequence of ginseng peptide gene was designed and synthesized by the solid phase phosphoramidiate method. Synthetic segments were isolated, pllrified and joined to the plasmid pUC19. E.icherichiu coli JM101 cells were transformed with above hybrid plasmids. AmpiciIBin resistant transformants were screened and identified by in situ colony hybridization and Southern blot techniques. Finally the gene sequencing was done by the Sanger dideoxy method using primer extension.