생약학회지 (Korean Journal of Pharmacognosy)

  • 제21권3호
  • /
  • Pages.205-209
  • /
  • 1990
  • /
  • 0253-3073(pISSN)
  • /
  • 2288-9299(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

조직배양(組織培養)한 시호근(柴胡根)의 Saikosaponin 함량(含量)

Saikosaponin Contents in Bupleurum falcatum Root Produced by Tissue Culture

  • 조필형 (한풍제약중앙연구소 생물공학연구실) ;
  • 성락선 (한풍제약중앙연구소 생물공학연구실) ;
  • 배형화 (한풍제약중앙연구소 생물공학연구실) ;
  • 소웅영 (전북대학교 자연과학대학 생물학과) ;
  • 조덕이 (전주우석대학 생물학과)
  • Jo, Pil-Hyung (Laboratory of Biotechnology, Hanpoong Central Research Center) ;
  • Seong, Rack-Seon (Laboratory of Biotechnology, Hanpoong Central Research Center) ;
  • Bae, Hyung-Hwa (Laboratory of Biotechnology, Hanpoong Central Research Center) ;
  • Soh, Woong-Young (Department of Biology, College of Natural Science, Chonbuk National University) ;
  • Cho, Duck-Yee (Department of Biology, Chonju Wooseok College)
  • 발행 : 1990.09.30
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초록

The study for the quantitative analysis of saikosaponin of Bupleurum falcatum root produced by tissue culture was attempted. The optimal concentration of 2,4-D was 0.1 mg/l for inducing the callus. The induction and growth of the adventitious root from the callus was obtained in the suspension culture containing MS basal medium supplemented with no plant growth regulators. The results of the quantitative analysis of saikosaponins by high performance liquid chromatography (HPLC) showed that the content of saikosaponin a and c was high in the one-year-old root from somatic embryos and that of saikosaponin d was remarkably high in three-months-old adventitious root from callus.

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