한국균학회지 (The Korean Journal of Mycology)
- 18권2호
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- Pages.77-83
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- 1990
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- 0253-651X(pISSN)
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- 2383-5249(eISSN)
느타리버섯속(屬)의 원형질체(原形質體) 융합률(融合率)에 영향(影響)을 미치는 요인(要因)
Factors Affecting Fusion Frequency of Pleurotus Protoplasts
- 유영복 (농촌진흥청 농업기술연구소 균이과) ;
- 김영태 (농촌진흥청 농업기술연구소 균이과) ;
- 변명옥 (농촌진흥청 농업기술연구소 균이과) ;
- 유창현 (농촌진흥청 농업기술연구소 균이과) ;
- 차동열 (농촌진흥청 농업기술연구소 균이과) ;
- 박영환 (농촌진흥청 농업기술연구소 균이과)
- Yoo, Young-Bok (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, R.D.A.) ;
- Kim, Yeong-Tae (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, R.D.A.) ;
- Byun, Myung-Ok (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, R.D.A.) ;
- You, Chang-Hyun (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, R.D.A.) ;
- Cha, Dong-Yeul (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, R.D.A.) ;
- Park, Yong-Hwan (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, R.D.A.)
- 발행 : 1990.06.30
초록
사철느타리버섯과 느타리버섯의 영양요구주로 원형질체 융합과정을 관찰하고, 융합에 영향을 미치는 여러 가지 요인을 분석한 결과는 다음과 같다. 원형질체에 polyethylene glycol을 혼합하였을 때 원형질체는 서로 응집되어 원형질막의 접착이 이루어졌으며, 0.6 M sucrose로 PEG를 제거한 후에 두 개 또는 다수의 원형질체가 융합되었다. 원형질체 융합에 크게 영향을 주는 요인은 PEG 농도, pH, 삼투압조정제였으며 원형질체의 농도, PEG 흔합시간, 원형질체 배양온도 및 시간, PEG 분자량,
Factors influencing the fusion frequency of protoplasts were investigated with auxotrophic mutants of Pleurotus florida and Pleurotus ostreatus. Immediately after the polyethylene glycol (PEG) solution was added, the protoplasts adhered firmly and shrank. During the subsequent dilution with 0.6 M sucrose, the protoplasts regained their normal size and larger bodies were observed. Interspecific heterokaryons were obtained by fusion of the nutritionally complementing protoplasts. Hyphae of the heterokaryotic fusants formed true clamp connections. The optimum conditions were a total of 1 to 15 million protoplasts per ml, 30% polyethylene glycol 8000 solution with adjustment to pH 8.0 and 0.6 M sucrose stabilized regeneration medium. Other parameters such as
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