Cloning of the MLS Antibiotics Inducible Resistance Gene and Its Control Mechanism -Inducible Resistance to MLS Antibiotics of pMB4 Plasmid Isolated from Streptococcus sp. TR-1-

MLS계 항생물질 유도 내성 유전자의 크로닝과 유전자의 조절기전 -Streptococcus sp. TR-1에서 분리한 pMB 4 Plasmid의 MLS계 항생물질 유도내성-

Streptococcus sp. TR-1 which has inducible resistance to MLS antibiotics was isolated from soil samples in Korea. Streptococcus sp. TR-1 was cultured in Lysis broth, then a plasmid was isolated by modified Elliker method. Bacillus subtilis UOTO277 was transformed with that plasmid. This result showed that the plasmid has the gene relating with inducible resistance to MLS antibiotics. It was named pMB4 and its size was determined about 2.4 Kb by results of digestion with various restriction enzymes. Restriction endonuclease cleavage site map of pMB4 plasmid was made by double digestion of the plasmid. pMB4 plasmid has different restriction endonuclease site map from the other plasmids that have been discovered in Streptococcus sp. so far. And it could be identified that pMB4 plasmid does not have homology with ermK of Bacillus licheniformis EMR but has homology with ermC of Staphylococcus aureus from the results of Southern hybridization.