알칼리성 Protease를 생성하는 알칼리성 세균 및 그 효소활성에 관한 연구

Studies on the Alkalophilic Bacteria Producing Alkaline Protease and its Enzyme Activities

Alkalophilic bacteria isolated from compost were selected, identified and tested for production of alkaline protease. The bacterium was tentatively assigned to Bacillus sp. based on the morphological, cultural and biochemical characteristics. The optimum pH of growth was 10 Galactose and Sodium glutamate enhanced the production of alkaline protease. The protease was most active at pH 11.0 and $60^{\circ}C$ and stable in the range of pH 5-11 and temp. $30^{\circ}-55^{\circ}C$. The protease was stabilized by the presence of calcium salts.