Clinical and Experimental Reproductive Medicine

  • 제16권2호
  • /
  • Pages.113-118
  • /
  • 1989
  • /
  • 2233-8233(pISSN)
  • /
  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지

생쥐 정자의 운동성에 미치는 Calcium 과 dbcAMP 의 영향

Effects of Calcium and dbcAMP on Sperm Motility in Mouse

  • 김문규 (한양대학교 자연과학대학 생물학과) ;
  • 오승훈 (한양대학교 자연과학대학 생물학과) ;
  • 계명찬 (한양대학교 자연과학대학 생물학과) ;
  • 윤현수 (한양대학교 자연과학대학 생물학과) ;
  • 이준영 (충북대학교 자연과학대학 생물학과)
  • Kim, Moon-K. (Department of Biology, College of Natural Sciences , Hanyang University) ;
  • Oh, Seung-H. (Department of Biology, College of Natural Sciences , Hanyang University) ;
  • Gye, Myung-C. (Department of Biology, College of Natural Sciences , Hanyang University) ;
  • Yoon, Hyun-S. (Department of Biology, College of Natural Sciences , Hanyang University) ;
  • Lee, Joon-Y. (Department of Biology, College of Natural Sciences, Chungbuk University)
  • 발행 : 1989.12.30
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초록

In order to study the effects of $Ca^{++}$ and dbcAMP on sperm motility, cauda epididyal spermatozoa of mouse were incubated in the media containing the various concentrations of $Ca^{++}$ and dbcAMP, and Its motility were observed and analyzed. The rate(%) of the sperm with forward motility which may be fertile, reached at the equilibrium state in 30-60 min. after the beginning of incubation. $Ca^{++}$ requirement for the maintenance of sperm motility obviously appeared in 3hrs. after the beginning of incubation. In the medium containing 0.01mM $Ca^{++}$, the rate of sperm forward motility was highest(30.6-40.6%), whereas in 10mM $Ca^{++}$ medium, the rate was rather reduced because of a severe agglutination phenomenon. On the other hand, 1mM dbcAMP in the medium was significantly effective for the maintenance of sperm forward motility.

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