Korean Journal of Veterinary Research (대한수의학회지)
- Volume 29 Issue 4
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- Pages.525-530
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- 1989
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Enumeration of Korean native goat erythrocytes (KGRBC)-rosette forming cells in peripheral blood of Korean cattle
재래산양 적혈구를 이용한 한우 순환 혈액내 rosette 형성 세포 정량
- Cheong, Ki-soo (Yeong Dong-Branch of Kangwon-Do Animal Health Laboratory) ;
- Kim, Neun-su (Yeong Dong-Branch of Kangwon-Do Animal Health Laboratory) ;
- Kim, Dong-hoon (Yeong Dong-Branch of Kangwon-Do Animal Health Laboratory) ;
- Kang, Myoung-dai (College of Veterinary Medicine, Chonbuk National University) ;
- Song, Hee-jong (College of Veterinary Medicine, Chonbuk National University)
- 정기수 (가축위생시험소 영동지소) ;
- 김년수 (가축위생시험소 영동지소) ;
- 김동훈 (가축위생시험소 영동지소) ;
- 강명대 (전북대학교 수의과대학) ;
- 송희종 (전북대학교 수의과대학)
- Received : 1989.07.26
- Published : 1989.10.28
Abstract
In order to enumerate the T-lymphocytes in bovine peripheral blood lymphocytes (PBL) by E rosette assay, KGRBC were treated with various concentrations of 2-aminoethylisothiouronium bromide(AET) and dextran(Dex), singly or in combination. To further standardize the assay, optimum concentration of AET- and/or Dex-treatment and incubation time for rosette forming cell(RFC) counts were determined. The levels of B-lymphocytes in the PBL were evaluated by erythzocyte-antibody(