Korean Journal of Veterinary Research (대한수의학회지)
- Volume 29 Issue 2
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- Pages.33-39
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- 1989
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Separation of monocytes from canine peripheral blood
개 말초혈액(末稍血液)에서 monocytes 분리(分離)
- Kim, Jeoung-bae (College of Veterinary Medicine, Chonnam National University) ;
- Lee, Bang-whan (College of Veterinary Medicine, Chonnam National University)
- Received : 1989.01.30
- Published : 1989.04.30
Abstract
Pure separation of various leukocytes is required for the assessment of their roles in immunological and phisiological function. In this study, pure separation of monocytes from canine peripheral blood was attempted. At first, mononuclear cells (PBMC) were separated by ficoll-hypaque gradient method and then monocytes were recovered from PBMC suspensions in sucrose gradient Sol. (PBMC-Sucrose), autologous plasma (PBMC-Plasma) and autologous serum (PBMC-Serum) incubated at