대한핵의학회지 (The Korean Journal of Nuclear Medicine)

대한핵의학회 (The Korean Society of Nuclear Medicine)
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Cyclosporine 방사면역측정법의 정도관리

Quality Control for Radioimmunoassay of Cyclosporine

  • 정재민 (서울대학교병원 핵의학과) ;
  • 서일택 (서울대학교병원 핵의학과) ;
  • 문대혁 (서울대학교 의과대학 내과학교실) ;
  • 정준기 (서울대학교 의과대학 내과학교실) ;
  • 이명철 (서울대학교 의과대학 내과학교실) ;
  • 조보연 (서울대학교 의과대학 내과학교실) ;
  • 고창순 (서울대학교 의과대학 내과학교실)
  • Jeong, Jae-Min (Department of Nuclear Medicine, Seoul National University Hospital) ;
  • Seo, Il-Tack (Department of Nuclear Medicine, Seoul National University Hospital) ;
  • Moon, Dae-Hyuk (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Chung, June-Key (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Lee, Myung-Chul (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Cho, Bo-Youn (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Koh, Chang-Soon (Department of Internal Medicine, College of Medicine, Seoul National University)
  • 발행 : 1989.11.13
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초록

According to the development of monoclonal antibodies against cyclosporine, it became available to replace the conventional polyclonal antibody method with new monoclonal antibody method to measure the blood level of cyclosporine by radioimmunoassay. We compared the results obtained by the two methods: polyclonal antibody and monoclonal antibody method. The results were obtained as follows: 1) We obtained mean value 137.3 ng/ml and CV 16.1% from plasma sample, and mean values 495.7 ng/ml and 1053.8 ng/ml and CVs 19.3% and 17.4% respectively from whole blood sample by polyclonal antibody method. 2) For the two control groups, 100 ng/ml 400 ng/ml each, we obtained that the CVs were 20.2% and 14.0% respectively from plasma sample, and 11 9% and 13.1% respectively from whole blood sample by monoclonal antibody method. In conclusion, we found that cyclosporine RIA was a relatively reliable method to measure blood or plasma concentration. Especially RIA using monocloanl antibody showed less degree of error in measurement compared to polyclonal antibody method.

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