Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 17 Issue 6
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- Pages.545-551
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- 1989
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Purification and Characterization of Thermotolerable Alkaline Protease by Alkalophilic Bacillus sp. No. 8-16
알칼리성 Bacillus sp. No.8-16의 내열ㆍ알칼리성 단백질 분해효소의 정제와 특성
- Bae, Moo (Department of Biology, Ewha Womans University) ;
- Park, Pil-Yon (Department of Biology, Ewha Womans University)
- Published : 1989.12.01
Abstract
Thermostable alkaline protease of alkalophilic Bacillus sp. No. 8-16 has been purified, and the properties of the enzyme investigated. The characteristic point of the organism used is especially good growth in alkaline and thermal condition. The alkaline protease of the strain No. 8-16 was purified from crude enzyme by acetone precipitation, CM-cellulose ion exchange chromatography, Sephadex G-100 and Sephadex G-75 gel filtration. Through the series of chromatograpies, the enzyme was purified to homogeneity with specific activity of 37 fold higher than that of the crude broth. Characteristics of the purified enzyme were as follow;
알칼리성 Bacillus속 8-16 균주의 내열ㆍ알칼리성단백질 분해효소를 정제하여 그 특성을 조사하였다. 본 균주의 알칼리성 protease를 아세톤침전, CM-셀룰로즈크로마토그래피, Sephadex G-100 및 G-75 젤 여과법으로 정제하였고 비활성이 37배되게 하여 단일단백질이 될 때까지 순수정제하였다. 이 효소는 7