생쥐 복수로부터의 단세포군 항체분리를 위한 크로마토그라피 분리정제 방법의 개발 Part II. 히드록실아파타이트 크로마토그라피 단일 단계만의 사용

Development of Chromatographic Downstream Processing for the Purification of Monoclonal Antibody from Ascites Fluid: Part II Use of Single Hydroxylapatite Chromatographic Step

In order to obtain monoclonal antibody from ascites fluid at sufficiently high purity using a single hydroxylapatite chromatography (HA) a further optimization on its operating variables was carried out. By adjusting the pH of the eluent, the sodium phosphate buffer, to 6.0 from 6.8 and adding CaCl$_2$to 1 mM at the column inlet, the elution molarities (M$_{elu}$) for the desired monoclonal antibody and contaminating proteins can be distinguished from each other with enough resolution. Previously these two groups of proteins co-eluted at the same time at pH 6.8 and without CaCl$_2$. This sin81e step hydroxylapatite chromatography yields the desired antibody pure enough for diagnostic use.