Abstract
Mass production of Spodoptera litura nuclear polyhedrosis virus (SINPV) was carried out on massively reared host insects. The yield of SINPV was maximal with $6.7{\;}\times{\;}10^9$ PIBs per larva on the 8th day post inoculation, when 5th instar larvae were inoculated with $1.1{\;}{\times}{\;}10^7$ PIBs per ml, and 2 g of artificial diet was sufficient for food consumption of a larva. The moribund larvae were more suitable for handling and mass production of virus than the completely dead larvae. The larvae, when treated with methoprene ($Manta^{\circledR}$), prolonged their larval period and consequently became bigger to result in higher yield(about 15%) of virus.
담배거세미나방핵다각체병바이러스대량생산연구에서, 5영충에 $1.1{\;}{\times}{\;}10^7다각체/ml$로 접종 후 8일에 수확했을 때 한 마리당 $6.7{\;}\times{\;}10^9$ 다각체를생산할 수 있었으며, 이때 통과는 한 마리당 약 2g의 인공통과로 충분하였다. 합성유약 호르몬인 methoprene ($Manta^{\circledR}$)을 처리했을 때 종영유충기간이 1~2일 연장되었으며, 바이러스 생산에 있어서도 무처리에 비해 약 15% 증가되었다.