생장조절제에 의한 인삼(Panax ginseng C. A. Meyer)의 기내 화아형성 조절

Control of In Vitro Flowering of Ginseng(Panax ginseng C. A. Meyer) by Growth Regulators

  • 이행순 (한국과학기술연구원 유전공학센터 식물세포생물학 연구실, 서울대학교 자연과학대학 식물학과)
  • 발행 : 1989.12.01

초록

Ginseng zygotic embryos, seedlings, and exised cotyledonary nodes were cultured on Murashinge and Skoog's(MS) medium, supplemented with 6-benzyladenine(BA) and gibberellic acid(GA3) to induce flower buds. As the concenteration of nitrogen compounds in MS medium was reduced to half of its strength, the flowering frequency of zygotic embryos increased up to 90%. The optimum concentration of sucrose in the medium for flowering of seedlings was 30-60 g/1. In all cases flower buds were formed on elongated axillary branches from the cotyledonary nodes, while the apices remained vegetative. When zygotic embryos and excised cotyledonary nodes were cultured on the medium, supplemented with all possible combinations of BA, GA3, and abscisic acid(ABA) of 5 $\mu$M indole-3-acetic acid(IAA) in the above combinations did not affect flowering. These results suggest that cytokinins, gibberellins, and inhibitors play primary, permissive, and preventive roles, respectively, in the induction of flowering of ginseng.

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