Abstract
Solid gelatin microcarrier with the size distribution between $100{\mu}{\textrm}{m}$ and $400{\mu}{\textrm}{m}$ was prepared for the attachment and growth experiment for anchorage-dependent animal cell lines, i.e., L 929 and BHK 21. The growth and the maximum cell densities on this gelatin based and polyacrylamide (PAA) microcarriers were compared with those on the commercial dextran based Cytodex 3 microcarrier. Both cell lines showed good comparable attachment and growth patterns on the above three microcarriers. The mouse fibroblast, L 929 showed about the same maximum cell density on PAA, gelatin and Cytodex 3 MC'S BUT BHK 21, the baby hamster kidney cell line, showed the best result on Cytodex 3, which was about $4\times10^6$ cells/ml with the microcarrier concentration of 10 g/1.
첼라틴을 이용하여 직경이 $100~400{\mu}{\textrm}{m}$의 분포를 가잔 구행 미 겁 담체를 만들어 부착성 동물세포들의 부착및 성 장정도를 측정하였다. 또 상업화된 Cytodex Ill와 실험실에서 제조된 Polyacrylamide 미립담체들을 가지고 세포 성장 정도를 비교검토하였다. DMEM 애지에서 L929 (mouse fibroblast)와 BHK 21(Baby Hamster Kidney)부착성 세포를 배양한 결과 L 929는 P AA, Cytodex ill에서 가장 좋은 성장을 보였다. 109/L의 Cytodex 농도에서 최대세포농도는 약 $4\times10^6$cells/ML 이였다.
