Radiation Oncology Journal
- 제7권2호
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- Pages.149-156
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- 1989
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- 2234-1900(pISSN)
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- 2234-3156(eISSN)
HeLa 세포의 Spheroid에 대한 방사선과 Platinum 유사체의 치사 효과
Lethal Effects of Radiation and Platinum Analogues on Multicellular Spheroids of HeLa Cells
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홍성언
(경희대학교 의과대학 치료방사선과학교실)
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Hong, Seong-Eon
(Department of Therapeutic Radiology, College of Medicine, Kyung Hee University)
- 발행 : 1989.12.01
초록
HeLa 세포의 spheroid를 배 양시켜 cis-platinum과 carboplatin으로 처리한 후 그 반응을 세포의 생존분획으로 분석하였다. 체외실험 model인 spheroid를 사용하여 platinum유사체의 약효와 방사선 감수성을 평가하고 약제에 대한 단층 세포와 spheroid의 감수성 차이를 세포-생존곡선에서 규명하기 위하여 본 실험을 시행하였다. Cis-platinum 농도-곡선에서 spheroid의
Multicellular tumor spheroids of HeLa cells have been grown in a static culture system. Samples of spheroids were exposed for 2 h to graded concentration of cis-platinum and its analogue, carboplatin, and then response assayed by survival of clonogenic cells. The purpose of present experiment is to clarify the effectiveness of these platinum compounds and to evaluate intrinsic radiosensitivity of cells using spheroids of HeLa cells as an experimental in vitro model. Variations of the drug sensitivity of monolayers as well as spheroids were also evaluated in cell-survival curves. In cis-platinum concentration-survival curve, there was a large shoulder extending as far as