한국식품과학회지 (Korean Journal of Food Science and Technology)
- 제21권4호
- /
- Pages.542-548
- /
- 1989
- /
- 0367-6293(pISSN)
AOT-Isooctane 역마이셀계를 이용한 단백질 분리에 관한 연구
A Study on Protein Separation with AOT-Isooctane Reverse Micellar System
- Rho, Young-Tae (Department of Food Engineering, Yonsei University) ;
- Kim, Young-Sug (Department of Food Engineering, Yonsei University) ;
- Cho, Hyung-Yong (Department of Food Engineering, Yonsei University) ;
- Pyun, Yu-Ryang (Department of Food Engineering, Yonsei University)
- 발행 : 1989.08.01
초록
AOT-Isooctane 역마이셀계를 이용한 단백질 추출 및 회수에 영향을 미치는 여러 가지 요인과 단백질 혼합물로부터의 선택적인 분리에 대해 연구 검토하였다. Lysozyme,
For the selective separation of proteins, the solubilization and desolubilization of proteins in sodium-di-2-ethylhexyl sulfosuccinate (AOT)-isooctane reverse micellar system were investigated. Protein solubilization increased with increasing the concentration of AOT to 200 mM and then decreased above that concentration. Protein was solubilized into reverse micelles in the pH range below the isoelectric Point of each protein, pH 4-10 for lysozyme and pH 5-6 for trypsin and