Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Production of Red Pigment by Using Protoplast Fusion of Monascus anka

Monascus 속간의 원형질체 융합에 의한 적색색소의 생산

  • Ryu, Beung-Ho (Department of Food Science and Technology, Kyungsung University) ;
  • Lee, Byeong-Ho (Department of Food and Nutrition, Dongeui University) ;
  • Park, Bub-Gyu (The Institute of Food Science and Microbiology, Kyungsung University) ;
  • Kim, Hee-Sock (Department of Food Science and Technology, Kyungsung University) ;
  • Kim, Hye-Sung (Department of Food Science and Technology, Kyungsung University) ;
  • Kim, Dong-Seuk (Department of Food Science and Technology, Kyungsung University) ;
  • Roh, Myung-Hoon (Department of Food Science and Technology, Kyungsung University)
  • 류병호 (경성대학교 식품공학과) ;
  • 이병호 (동의대학교 식품영양학과) ;
  • 박법규 (경성대학교 종합식품연구소) ;
  • 김희숙 (경성대학교 식품공학과) ;
  • 김혜성 (경성대학교 식품공학과) ;
  • 김동석 (경성대학교 식품공학과) ;
  • 노명훈 (경성대학교 식품공학과)
  • Published : 1989.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study aims at producing red pigment effectively by using protoplast fusion. Auxotrophic mutants of M. anka, $4478-27-37(Thi^-,\;Met^-),\;4478-27-62(Thi^-,\;Arg^-)$ were derived from M. anka, 4478-27. M. anka, $6540-185-24\;(Pan^-,\;Leu^-)$ and M. anka, $6540-185-72\;(Arg^-)$ were derived from M. anka, 6540-185. The optimal conditions of protoplast fromation were at time by using mixtures of chitinase (100mg/ml), cellulase (5mg/ml) and ${\beta}-glucuronidase\;(5mg/ml)$. The protoplast of those auxotrophic mutants were fused effectively, in the solution of 30% PEG $6,000,001M-CaCl_2$, 0.05M-glycine, pH 6.0. Fusion frequencies were 0.70%-0.85%. Fusants, No. 14 and 42 produced highest red pigment among them.

Monascus 균주의 적색색소의 최적 생산조건을 규명하기 위하여, Monascus anka IFO 4478과 IFO 6540의 영양요구성 돌연변이주인 M. anka, 4478-27-37 $(Thi^-,\;Met^-),\;4478-27-62\;(Thi^-,\;Arg^-)$와 M. anka, 6540-185-24 $(Pan^-,\;Leu^-)$ 6540-185-72$(Arg^-)$의 균주를 이용하여 세포융합을 시도하였다. Protoplasts의 생성은 chitinase (10mg/ml), cellulase (5mg/ml) 및 ${\beta}-glucuronidase\;(5mg/ml)$의 혼합처리가 효과적이었고, 융합조건은 protoplasts를 30% PEG 6.000, $0.01M-CaCl_2$ 및 0.05M-glycine, pH 6.0으로 처리했을 때 융합빈도는 0.7%-0.85%이였다. 융합주 중에서 No. 14와 42의 색소 생산능이 가장 높았다.

Keywords