The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Transfer of Isolated Nuclei from Agrocybe aegerita Mycelia into Pleurotus florida Protoplasts

사철느타리버섯 원형질체내(原形質體內) 버들볏짚버섯 핵(核)의 전이(轉移)

  • Yoo, Young-Bok (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA) ;
  • Shin, Pyung-Gyun (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA) ;
  • Kim, Han-Kyoung (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA) ;
  • Byun, Myung-Ok (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA) ;
  • You, Chang-Hyun (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA) ;
  • Cha, Dong-Yeul (Applied Mycology and Mushroom Division, Agricultural Sciences Institute, RDA) ;
  • Chang, Kwon-Yawl (Department of Agronomy, College of Agriculture, Gyeongsang National University)
  • 유영복 (농촌진흥청 농업기술연구소 균이과) ;
  • 신평균 (농촌진흥청 농업기술연구소 균이과) ;
  • 김한경 (농촌진흥청 농업기술연구소 균이과) ;
  • 변명옥 (농촌진흥청 농업기술연구소 균이과) ;
  • 류창현 (농촌진흥청 농업기술연구소 균이과) ;
  • 차동열 (농촌진흥청 농업기술연구소 균이과) ;
  • 장권열 (경상대학교 농과대학 농학과)
  • Published : 1989.09.30
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Abstract

The transfer of isolated nuclei from Agrocybe aegerita mycelia of wild type into Pleurotus florida protoplasts of auxotroph was induced with polyethylene glycol. The type 1 of nuclear transfer products was spontaneous segregants of both parental morphology of colony. Hyphae of A. aegerita type had clamp connections while that of P. florida type lacked. Type 2 was main products of nuclear transfer which formed true clamp connections. Type 3 was clampless products. They all produced primordia and developed basidiocarps similar to Agrocybe aegerita. A comparison of nuclei transfer products was made using isozyme analyses of alcohol dehydrogenase, esterase, lactate dehydrogenase and peroxidase. Isozyme band patterns of some type 2 strains produced a new band. Band patterns from mycelial extracts of the other strains could be characterized by parental bands.

야생형(野生形)이며 self-fertile인 버들볏짚버섯(버들송이) 균사체(菌絲體)로부터 분리(分離)한 핵(核)을 영양요구주(營養要求株)이며 self-sterile인 사철느타리버섯 원형질체내(原形質體內)에 polyethylene glycol+$CaCI_2$+glycine 용액으로 섭입(攝入)하였다. 100균주(菌株)의 핵전이주(核轉移株) 검정(檢定)으로 양친(兩親)이 균총분리(菌叢分離)되어 버들볏짚버섯친(親) 형태(形態)에는 클램프 연결체(連結體)가 균사(菌絲)에 있고, 사철느타리버섯친(親) 형태(形態)에는 클램프 연결체(連結體)가 없는 것이 3균주(菌株), 균총형태(菌叢形態)가 안정성(安定性)이며 클램프 연결체(連結體)가 균사(菌絲)에 형성(形成)된 것이 55균주(菌株), 균총형태(菌叢形態)가 안정성(安定性)이며 클램프 연결체(連結體)가 균사(菌絲)에 존재(存在)하지 않는 것이 42균주(菌株)였다. 이들 핵전이주(核轉移株)는 모두 원기(原基) 형성(形成)되어 자실체(子實體)로 성숙되었으며 그 형태(形態)는 버들볏짚버섯과 거의 유사(類似)하였다. 핵전이주(核轉移株)를 전기영동법으로 alcohol dehydrogenase, esterase, lactate dehydrogenase, peroxidase의 동위효소(同位酵素) 분석(分析)으로 비교하였는데 클램프 연결체(連結體)를 가지는 형태(形態)의 몇 균주(菌株)가 새로운 band를 형성(形成)하였으며 다른 균주(菌株)들은 양친주(兩親株)에서 유래된 것이었다.

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