Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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A Cytochemical Study on the Acid Phosphatase of Neurons of Developing Chick Embryo Brain

계배 뇌 발생에 있어서 신경세포의 Acid Phosphatase 변화에 관한 효소화학적 연구

  • Koh, Ki-Seok (Dept. of Biology, College of Science, Yonsei University) ;
  • Shin, Chu-Og (Dept. of Biology, College of Science, Yonsei University) ;
  • Yoo, Chang-Kyu (Dept. of Biology, College of Science, Yonsei University) ;
  • Choe, Rim-Soon (Dept. of Biology, College of Science, Yonsei University)
  • 고기석 (연세대학교 이과대학 생물학과) ;
  • 신주옥 (연세대학교 이과대학 생물학과) ;
  • 유창규 (연세대학교 이과대학 생물학과) ;
  • 최임순 (연세대학교 이과대학 생물학과)
  • Published : 1988.12.01
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Abstract

The purpose of this study was to investigate the differentiation and degeneration of neurons in developing chick embryo. The activity of acid phosphatase(ACP) was measured and cytochemical study of ACP and ultrastructural changes were observed in prosencephalon, mesencephalon and rhombencephalon from day 4 to day 19 of incubation. As a result, the activity of ACP of all brain region was tend to increase from day 4 to day 19. On day 13, activities of ACP of mesencephalon and rhombencephalon were increased greatly and activity of ACP was decreased each region on day 17. On electron microscopic examination, the reaction product of ACP were localized at GERL complex, lysosome, Golgi body and vacuoles of neurons. Morphologically, disrupted nuclear envelope, mitochondrial destruction, vacuolization and ribosomal crystalization were observed.

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