Bulletin of the Korean Chemical Society

  • 제9권1호
  • /
  • Pages.44-48
  • /
  • 1988
  • /
  • 0253-2964(pISSN)
  • /
  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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Evaluation of a Radical Mechanistic Probe for NADH-dependent Horse Liver Alcohol Dehydrogenase Reactions by Computer Graphics Modeling

  • Chung Sung Kee (Smith Kline & French Laboratories 1500 Spring Garden St) ;
  • Chodosh Daniel F. (Institute of Science & Technology)
  • 발행 : 1988.02.20
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초록

The feasibility of the reduction of nortricyclanone (1) as a chemical probe for testing the proposed radical mechanism for NAD-dependent horse liver alcohol dehydrogenase (HLADH) reactions has been examined using computer graphics modeling. The resutls of this study suggest that the radical ring-opening of this probe molecule may involve too substantial a geometry reorganization for the molecule to serve as a chemical probe in detecting the possible presence of the radical intermediates in the HLADH reactions. This result suggests that one should exercise caution in extrapolating results obtained from chemically based radical probes in the solution phase to the topologically constrained systems such as enzyme-substrate reactions.

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