Assay of Cellobiohydrolnse by Column Single Immunodiffusion and Enzyme tinted Immunosorbent Assay

면역화학적 방법에 의한 Cellobiohydrolase 정량

Antibody against cellobiohydrolase purified from Trichoderma viride had been obtained by injection to rabbit. The antibody had a high specificity against the cellobiohydroase evidienced by absence of immunological reaction to other isozymes from Trichoderma viride. Assay limit of cellobiohydrolase was 1-10 $\mu\textrm{g}$ by column single immunodiffusion and by enzyme linked immunosorbent assay, it was 10-140 ng and 100-1200 pg when the dilution of antibody was 10$^{-6}$ and 10$^{-5}$, respectively.

Trichoderma viride가 분비하는 cellobiohydrolase를 항원으로 사용하여 이 섬유소 분해효소에만 특이적인 항체를 얻을 수 있었다. 이 항체를 이용하여 column single immunodiffusion 방법에 의해서 cellobiohydrolase의 농도 2-10 $\mu\textrm{g}$ 범위 내에서는 직선적으로 정량 할 수 있었고, ELISA 방법을 사용할 때는 $10^6$의 항체 희석시는 40-110ng, $10^5$ 항체 희석시는 100-1,200pg 농도범위의 cellobiohydrolase 를 정량 할 수 있었다.