Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Incidence of Plasmids in Marine Bacteria Isolated from the Bunker-C Oil Enriched Culture

Bunker-C유 집식배양으로부터 해양세균 Plasmid의 분포

  • Park, In-Sick (Department of Biological Science and Technology, National Fisheries University of Pusan) ;
  • Park, Jung-Youn (Department of Biological Science and Technology, National Fisheries University of Pusan) ;
  • Jin, Deuk-Hee (Department of Biological Science and Technology, National Fisheries University of Pusan) ;
  • Hong, Yong-Ki (Department of Biological Science and Technology, National Fisheries University of Pusan)
  • Published : 1988.08.01
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Abstract

Samples used for the enrichment culture were collected from the sea water of suspected chronic petroleum contamination in the vicinity of Pusan, Chungmu and Ulsan ports, Korea. Alkaline Iysis and agarose gel electrophoresis techniques were employed to screen these isolates for the presence of plasmid DNA. There were n little differences in the percentage of isolates containing plasmids between sampling sites of unpolluted sen water (22％) and polluted son water (25％). Bacterial isolates taken from the Bunker-C oil enriched culture showed significantly more plasmid incidence (29％). About two thirds of strains grown on a variety of hydrocarbons were Gram negative strains of which 33％ contained one or more plasmids. Multiple plasmids were observed in 23％ of the plasmid-carrying strains. Forty one percent of the plasmids detected were estimated to have a mass of 20 kb or more.

우리나라 연근해역의 해양오염중 주종을 이루는 Bunker-C유를 대상으로 부산 및 충무, 울산항구의 해수를 균원으로 접종하여 enrichment culture시켰다. 이와 같은 혼합배양 해양세균들의 plasmid DNA분포를 agarose gel 전기영동상에서 조사하여 보았다. 우선 오염되지 않은 해수로부터 분리한 세균들의 Plasmid 분포(22%)와 만성적으로 유류오염이 예상되는 각 항구의 세균 plasmid 분포(25%)에는 조금 차이는 있었으며 enrichment culture중에는 29%정도로서 plasmid 분포비율이 증가하였다. 각종 탄화수소 화합물들에 성장하는 세균중 약33%는 plasmid룰 함유하고 있었으며, 또 약 62%정도는 Gram 음성균이었다. Plasmid 함유 세균중 23% 정도는 2종 이상의 plasmid를 갖고 있으며, 41%의 plasmid는 20kb 이상의 크기를 가지고 있는 것으로 나타났다.

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