초록
성장속도가 빠르고포지형성이 우수한 방사균 일주를 토양에서 분리한 뒤 분리균의 특성을 조사한 결과 세포외 단백질 분해 효소가 중성과 알카리성의 두 종류가 생성되었으며 $\beta$-lactamase도 생성함을 알았다. 이 균주를 acriflarin 또는 NTG로서 처리하여 얻은 변이주는 포자의 형성, $\beta$-lactamase의 생성 및 protease의 생합성 능력이 소실 또는 크게 저하되었다. 일단계의 변이주 취득에서 동시에 형질의 변화가 다양하게 나타난 원인을 규명한 결과 호알카리성 protease의 생합성이 크게 저하되었음을 알았다. 따라서 방선균에서 포자형성과 호알카리성 protease의 활성이 일정한 연관성이 있을 것으로 판단되었다.
The aims of the present studies were to understand the physiolosical and genetic characters of Streptomyces sp. isolated from soil. It revealed that Streptomyces sp. SMF301 had very fast growth rate and produced extracellular protease and heavily sporulated on rich media. It also showed $\beta$-lactamase activity and pigment production. Nonsporulating mutants were isolated after NTG or acriflavin treatment and their characters were compared with the parent strain. It was found that the mutants obtained by acriflavin treatment and ghier characters were compared with the parent strain. It was found that the mutants obtained by acriflavin treatment lost the pigment formation and $\beta$-lactamase production. Protease actibity of the mutant was lowered and the pH optimum was changed toward neutral. It was found that the changes were resulted from the reduction of alkaline protease biosynthesis in the bald mutant. Therefore it is considered that sporulation, pigment formation, $\beta$-lactamase production, and alkaline protease production in Streptomyces sp. might be controlled with a closely related relationship.
