한국동물학회지 (The Korean Journal of Zoology)
- 제31권3호
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- Pages.177-184
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- 1988
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- 0440-2510(pISSN)
북방산 개구리(Rana dybowskii)의포의 프로젝트론 생서에 대한 cAMP의 조절작용
Role of cAMP in the Regulation of Progesterone Production and Secretion by Frog (Rana dybowskii) Follicles in vitro
초록
북방산개구리의 여포를 인공배양하면서 여포의 progesterone(p
The pattern of progesterone production and secretion of frog(R. dybowskii) follicles was investigated in follicle culture in vitro. Involvement of cAMP in the regulation of the steroid production by the follicles was also investigated by manipulating endogeneous cAMP level with forskolin and/or 3-isoburyl- 1 - methylxanthine(IBMX). Endogeneous follicular progesterone level increased rapidly in one hour of culture by treatment of frog pituitary homogenate(FPH) and reached peak level at 2 hours or later. But the absolute amount of progesterone produced (60-300 pg/follicle) or the peak time of the honnone level was different between individual animals. Basal level of progesterone in untreated sister follicles was very low (around 10 pg/follicle) and nearly undetectable in most cases regardless of culture lime. Secretion level of progesterone by the follicles obtained by measunng the honnone in the culture media was just the reflection of the intrafollicular level. Exogeneously added forskolin, an adenylate cyclase stimulator, and/or IBMX, a phosphodiesterase inhibitor, could mimic FPH action in terms of progesterone production and secretion. Thus, it seems clear that FPH regulates progesterone production via cAMP system in the follicle cells.