Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Production of Extracellular Protein from Bacillus sp.

Bacillus sp.에 의한 균체외 단백질의 생산

  • Lee, Jae-Sook (Department of Food Science and Technology, Chungnam National University) ;
  • Kim, Chan-Jo (Department of Food Science and Technology, Chungnam National University) ;
  • Lee, Jong-Soo (Department of Food Science and Technology, Chungnam National University)
  • 이재숙 (충남대학교 식품가공학과) ;
  • 김찬조 (충남대학교 식품가공학과) ;
  • 이종수 (충남대학교 식품가공학과)
  • Published : 1988.06.30
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Abstract

Extracellular protein was produced from bacterium, which was classified as a strain belong to the genus Bacillus. This bacterium produced 1.4mg/ml of extracellular protein when grown on chemically defined medium containing 3.0% of glucose, 1.3% of urea and 2.5% of calcium carbonate at $30^{\circ}C$ for 48 hours. The addition of $250{\mu}g/ml$ of cephalexin was effective for it and was produced 2.0mg/ml of extracellular protein for 96 hours.

미생물에 의한 단백질 생산과 그의 분비기작에 관한 기초 자료를 얻고자 토양으로 부터 균체외 단백질 생산균주를 분리하고 동정한 결과 Bacillus sp.로 추정 되었다. 선정균주를 3.0%의 glucose와 1.3%의 urea를 첨가한 chemically defined medium에 접종하여 $30^{\circ}C$에서 48시간 배양하였을때 1.2mg/ml의 단백질을 생산 하였고 $CaCO_3$의 첨가로 단백질 생산이 촉진 되었으며 $250{\mu}g/ml$의 cephalexin를 첨가하여 96시간 배양 하였을때 2.0mg/ml의 단백질을 생산 하였다.

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