Studies on Antimicrobial Activities and Safety of Natural Naringin in Korea

한국산 천연 Naringin의 항균작용 및 안전성에 관한 연구

To investigate the antimicrobial activities and safety of natural naringin, it was isolated with methanol from peels of Citri fructus. Its hydrolysate, naringenin was obtained by hydrolysis of naringin. In the antimicrobial activities of two components against eleven species of bacteria and eleven species of Fungi were examined by serial dilution method. Its result appeared to the minimal inhibitory concentration(MIC) and the antimicrobial activities of naringin and naringenin were compared. Naringenin showed considerably high order of activities against bacteria. There were no effect against Fungi $(MIC>100{\mu}g/ml)$. In the safety tests of naringin, examined for 50% lethal dose, Blood clinical chemical tests and organ tissue tests. The results showed that 50% lethal does in mice was 1,650 mg/kg. The experiments of administration in rats showed that there were no changes in blood clinical chemical future and organ tissue as control.

시판 감귤의 과피에서 naringin을 분리하고 가수분해하여 얻은 naringenin의 항균시험을 실시한 바 세균에서 naringin은 gram 양성균보다 gram 음성균이 비교적 강한 항균력을 나타내었으나 naringenin은 그 반대 현상을 나타내었다. 또 naringin과 naringenin의 항균력을 비교하면 전체적으로 naringenin이 매우 강한 항균작용이 있다. 안전성 시험에서 $LD_{50}$은 1650 mg/ kg이고 헐액임상화학적 검사에서 대조군과 비교하여 아무런 이상을 발견할 수 없었으며 병리조직학적 검사에서의 현미경 관찰에서도 대조군과 같았으며 투여군에 대해 조직학적 병변이 나타나지 않았으므로 안전성이 없다고 사료된다.