Separation of Amino Acid Enantiomers by Gas Chromatography (III) -(S)-5-isobutyl-$N^3$-phenyl-2-thiohydantoin as chiral stationary phase-

가스 크로마토그라피에 의한 아미노산 광학 이성질체의 분리(III) -(S)-5-isobutyl-$N^3$-phenyl-2-thiohydantoin의 광학 활성 고정상으로의 응용-

The resolution of N-trifluoroacetyl (N-TFA) ter-isopropylesters of Ala, Ile, Leu, Val and Phe by gas chromatography on the (S)-5-isobutyl-$N^3$-phenyl-2-thiohydantoin as stationary phase was investigated. The phase was employed at several column temperatures $(140^{\circ}C{\sim}200^{\circ}C)$ and the separation factors were $1.18{\sim}1.45$ range for five amino acid enantiomers. The possible mechanism of chiral recognition was investigated by NMR technique and the association constant$(K_c)$ was calculated as 201.3(r=0.98) for alanyl derivative.