Korean Journal Plant Pathology (한국식물병리학회지)
- Volume 3 Issue 2
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- Pages.124-130
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- 1987
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- 0256-8608(pISSN)
Purification and Cell Wall Regeneration of Protoplasts from Pyricularia oryzae Cav.
도열병균의 원형질체 나출 및 세포벽 재생
- Han S. S. (Department of Plant Pathology, Agricultural Sciences Institute) ;
- Lee Y. H. (Department of Plant Pathology, Agricultural Sciences Institute) ;
- Yoo J. D. (Department of Plant Pathology, Agricultural Sciences Institute) ;
- Lee E. J. (Department of Plant Pathology, Agricultural Sciences Institute)
- 한성숙 (농촌진흥청 농업기술연구소 병리과) ;
- 이영희 (농촌진흥청 농업기술연구소 병리과) ;
- 유재당 (농촌진흥청 농업기술연구소 병리과) ;
- 이은종 (농촌진흥청 농업기술연구소 병리과)
- Published : 1987.05.01
Abstract
The optimum conditions for protoplast formation and regeneration from Pyricularia oryzae Cav. were selected as follows. As a basic solution, 0.02M potassium phosphate buffer solution plus 0.6M KCl adjusted to pH 5.2 with 1N HCl was used. A mixture of enzyme combinations with 20mg Cellulase R-l0/ml, 5mg Macerozyme R-l0/ml and l0mg Driselase/ml used as a lytic enzyme showed better lytie effect than any single enzyme treatment for protoplast formation. Two-day-old mycelia of P. oryzae grown in the mixture of three lytie enzyme solution at
수도 도열병균(Pyricularia oryzae)의 균사체로부터 다량의 원형질체의 분리 및 세포벽 재생을 하기 위하여 필요한 몇가지 조건을 선발하였다. 나출을 위하여 기본용액으로 0.02M potassium phosphate buffer와 pH 5.2, 0.6M KCl로 삼투압을 조절하였고 분해효소는 ml당 각각 20mg Cellulase R-10, 5mg Macerozyme $-10, 10mg Driselase를 사용하였는데 각각의 단독처리구보다 3가지 효소의 복합처리구에서 원형질체 나출 정도가 우수하였다. 또한 선발된 복합효소액에 2일간 액체배양된 어린 균사체를 3시간,