Journal of Preventive Medicine and Public Health

The Korean Society for Preventive Medicine (대한예방의학회)
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Effects of Anticancer Agents on Cell Cycle Kinetics and Sister Chromatid Exchanges in Cultured Human Lymphocytes

항암제(抗癌劑)가 배양임파구(培養淋巴球)의 세포분열주기(細胞分裂週期) 및 자매염색분체교환(姉妹染色分體交換)에 미치는 영향(影響)

  • Hwang, In-Dam (Department of Preventive Medicine and Public Health, College of Medicine Chonbuk National University) ;
  • Ki, No-Suk (Department of Preventive Medicine and Public Health, College of Medicine Chonbuk National University) ;
  • Park, Won-Kihl (Department of Preventive Medicine and Public Health, College of Medicine Chonbuk National University) ;
  • Kim, Young-Oh (Department of Preventive Medicine and Public Health, College of Medicine Chonbuk National University) ;
  • Lee, Jeong-Sang (Department of Preventive Medicine and Public Health, College of Medicine Chonbuk National University)
  • 황인담 (전북대학교 의과대학 예방의학교실) ;
  • 기노석 (전북대학교 의과대학 예방의학교실) ;
  • 박원길 (전북대학교 의과대학 예방의학교실) ;
  • 김영오 (전북대학교 의과대학 예방의학교실) ;
  • 이정상 (전북대학교 의과대학 예방의학교실)
  • Published : 1987.05.01
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Abstract

Sister chromatid exchanges (SCEs) observed by means of bromodeoxyuridine substitution and fluorescence plus Giemsa (FPG) technique were proposed as a sensitive and quantitative assay for mutagenicity and cytotoxicity in short-term cultures of phytohaemagglutinin (PHA)-stimulated human lymphocytes. Therefore, this study was carried out to investigate the relation between anticancer agents and cytotoxic effects. Chromosomal analysis was performed on metaphase cells that had divided one, two, or three or more times after treatment for SCEs, mitotic indices (MI) and cell cycle kinetics by FPG technique. The results indicate that anticancer agents led to a dose dependent increase in SCE frequency except methotrexate. But, highly inhibited mitotic indices and delayed cell cycle kinetics were observed except for cyclophosphamide. The author suggest that the difference of SCE frequency is due to the differences in the cytotoxic action of anticancer agents, but although the induction of SCEs has a correlation with cell cycle delay, in some cases the induction of SCEs is not always related to cell cycle delay because of different cytotoxic action of anticancer agents.

항암제와 세포독성과의 관계를 알아보고자 인혈배양 임파구에서 SCE빈도, 세포분열지수 및 세포분열주기변동을 조사한 결과는 다음과 같다. 1) 항암제 농도증가에 따라 SCE빈도는 대조군에 비해 유의하게 증가되었으며 (P<0.01), methotrexate에서는 유의성이 인정되지 않았다(P>0.05). 2) 세포분열지수는 cyclophosphamide를 제외한 공시 항암제에 대하여 현저하게 감소되었다. 3) 항암제의 농도증가에 따라 세포분열주기는 현저하게 지연되었으며 고농도에서는 현저한 세포분열 억제로 정상적인 세포분열이라 할 수 없는 강한 세포독성효과를 보였다. 이 결과는 알킬계 약제가 다른 항암제에 비해 강한 SCE 유발제이며 SCE유발과 세포분열주기 지연과는 깊은 상관성이 인정되지만 methotrexote경우에서는 그 상관성을 인정할 수 없었다. 이는 SCE유발과 세포분열주기지연이 서로 다른 기전에 의해서도 나타남을 제시해주고 있다.

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