대한수의학회지 (Korean Journal of Veterinary Research)

  • 제27권1호
  • /
  • Pages.41-45
  • /
  • 1987
  • /
  • 2466-1384(pISSN)
  • /
  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지

Phytomitogen에 의한 기니픽 임파구의 Blast Transformation: II. 유사분열에 미치는 혈청농도와 배양시간의 효과

Phytomitogen Induced Blast Transformation of Guinea Pig: I. Effect of Concentration of Serum and Time of Incubation on the Uptake of 3H -Thymidine

  • 김종수 (경상대학교 농과대학 수의학과)
  • Kim, Jong-shu (Department of Veterinary Medicine, College of Agriculture, Gyeongsang National University)
  • 투고 : 1987.01.21
  • 발행 : 1987.04.30
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초록

The present study was carried out to investigate the optimal conditions on blastogenesis of guinea pig lymphocytes. A microculture system in conjuction with a semiautomatic multiple sample harvester was used to study the in vitro optimal condition of guinea pig lymphocytes. Careful analysis of lymphocyte transformation to concanavalin A (Con A) and lipopolysaccharide (LPS) mitogen determined optimal conditions as: (a) 10% fetal bovine serum in RPMI-1640 medium (b) 48-hour culture period.

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