우리나라 토착대두근류균(土着大豆根瘤菌)의 분포상태(分布狀態)와 생리(生理) 및 생태학적(生態學的) 특성(特性) -제(第)IV보(報) 토착대두근류균(土着大豆根瘤菌)의 질산환원(窒酸還元) 및 균체단백질(菌體蛋白質) 특성(特性)

Physiological and Ecological Characteristics of Indigenous Soybean Rhizobia Distributed in Korea -IV. Dissimilartory Nitrate Reduction and Protein Characteristics of Indigenous Soybean Rhizobia

전보(前報)에 이어 토양(土壤)의 특성(特性)이 상이(相異)한 대두재배지(大豆栽培地)로 부터 토착대두근류균(土着大豆根瘤菌)을 분리(分離)하여 유리(遊離) 상태(狀態)에서 질소환원작용(窒素還元作用)과 균체단백질(菌體蛋白質)의 전기영동(電氣泳動) pattern 상의 차이(差異)를 1차원(次元) 및 2차원(次元) 전기영동상(電氣泳動像)으로 분석(分析)한 결과(結果)는 다음과 같다. 1. 토착대두류균(土着大豆瘤菌)의 생육형(生育型) 및 이화적(異化的) 질산환원작용(窒酸還元作用)을 조사(調査)한 결과(結果) 4개군(個群)으로 구분(區分)되었다. 즉(卽) 총(總) 87개(個) 검정균주(檢定菌株)중 fast-grower는 38.7%, slow-grower는 61.3%로서 fast grower 보다 많았다. 2. 이들 각(各) 생육형간(生育型間)에는 탈질형(脫窒型)과 비탈질형(非脫窒型)(질산호흡형(窒酸呼吸型))으로 구분(區分)되었으나, 질산환원효소결여형(窒酸還元酵素缺如型)은 나타나지 않았다. fast-grower와 slow-grower 간(間)에 탈질형(脫窒型)은 전자(前者) 31%, 후자(後者)에서 89%, 그리고 비탈질형(非脫窒型)은 전자(前者) 69%, 후자(後者)에서 11%로 나타났다. 3. 토착대두근류균(土着大豆根瘤菌)의 균체단백질(菌體蛋白質)의 특성(特性)은 1차원(次元) 및 2차원(次元) 전기영동상(電氣泳動像)에서 fast-grower와 slow-grower의 양생육형간(兩生育型間)에는 차이(差異)를 볼수 있었으나, 탈질형(脫窒型)과 비탈질형간(非脫窒型間)에는 현저(顯著)한 차이(差異)를 나타내지 않았다.

In order to find out the effectiveness of nitrogen fixation in rhizobia-legume symbiotic relationship, ecological and physiological characteristics of indigenous rhizobia distributed in Korean soils, that is, dissimilatory nitrate reduction patterns of indigenous soybean rhizobia isolated from four different soils, and differences in one-and two-dimensional polyacrylamide gel electrophoretic pattern of proteins among the each subgroups of Bradyrhizobium japonicum and Rhizobium fredii, were investigated. The results were summarized as follows: 1. The indigenous soybean rhizobia isolated from four different soils could be classified into 4 groups depending on growth rate and dissimilatory nitrate reduction pattern, that is, $S_1$ (slow-grower; Bradyrhizobium japonicum and nitrate denitrifier), $S_2$ (slow-grower; Bradyrhizobium japonicum and nitrate respirer), $F_1$ (fast-grower; Rhizobium fredii and denitrifier), and $F_2$ (fast-grower; Rhizobium fredii and nitrate respirer). 2. The population ratio of fast- to slow-growing R. japonicum was 39% to 61%, and the ratio of denitrifier to nitrate respirer was 31% to 69% and 89% to 11% in fast and slow-grower, respectively. Some differences were observed between fast- and slow-growing R. japonicum but no significant difference was observed between denitrifier and nitrate respirer within same growth type by one and two dimensional SDS-polyacrylamide gel electrophoretic patterns.