Applied Microscopy
- Volume 17 Issue 1
- /
- Pages.83-97
- /
- 1987
- /
- 2287-5123(pISSN)
- /
- 2287-4445(eISSN)
Fine Structures of the Enteroendocrine Cells in the Duodenal Mucosa of the Hedgehog, Erinaceus koreanus
고슴도치 십이지장 점막의 장내분비세포의 미세구조
- Choi, Wol-Bong (Dept. of Anatomy, Faculty of Medicine, Hallym University) ;
- Won, Moo-Ho (Dept. of Anatomy, Faculty of Medicine, Hallym University) ;
- Seo, Ji-Eun (Dept. of Biology, College of Natural Sciences, Ewha Womans University)
- Published : 1987.05.01
Abstract
In order to discriminate the enteroendocrine cell types in the mucosal epithelium of the normal duodenum of the Korean hedgehog (Erinaceus koreanus). The tissues were fixed in the mixture of 1% paraformaldehyde and 1% glutaraldehyde in phosphate buffer (pH 7.2), and postfixed in 2% osmium tetroxide (phosphate buffer, pH 7.2). They were embedded in Araldite, and the ultrathin sections were made by LKB-V ultratome following the inspection of semithin sections stained with toluidine blue-borax solutions. Ultrathin sections contrasted with uranyl acetate and lead citrate were observed with JEM 100B electron microscope. At least six types of enteroendocrine cells distributed in the mucosal epithelium of the duodenum were identified according to their morphological characteristics mainly based on the size, shape, number and electron density of the secretory granules. Type I cells had moderately developed organelles. The secretory granules were pleomorphic (
Keywords