한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제15권5호
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- Pages.352-355
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- 1987
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Clostridium thermocellum으로 부터 새로운 type I I 제한효소 Cth I 의 분리
A New Restriction Endonuclease from Clostridium thermocellum
- Choi, K.D. (Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology) ;
- Kim, Kitae (Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology) ;
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Yoo, Ook-Joon
(Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology)
- 발행 : 1987.10.01
초록
새로운 제한효소인 Cth I을 Clostridium thermocellum ATCC 27405로부터 분리하여, 그 효소의 생화학적 특성을 연구하였다. 이 효소는 Bcl I endonuclease의 isoschizomer로서 5'-TGATCA-3'를 인식한다. Cth I endonuclease는 섭씨 60도의 높은 온도에서 잘 작용하며, 10mM까지의 저농도의 NaCl과 MG
The isolation and characterization of type II restriction endonuclease from Clostridium thermocellum ATCC 27405 were described. This enzyme (Cth I endonuclease) is an isoschizomer of Bcl I endonuclease recognizing 5'-TGATCA-3'. Cth I endonuclease requires MG