Rhizobium trifolii의 스트렙토마이신 내성 돌연변이주의 특성

Studies on streptomycin resistant mutant strains of rhizobium trifolii

  • 신종희 (고려대학교 이과대학 생물학과) ;
  • 허연주 (고려대학교 이과대학 생물학과) ;
  • 이영록 (고려대학교 이과대학 생물학과)
  • 발행 : 1987.12.01

초록

Some streptomycin resistant strains of Rhizobium trifolii having nodulation ability were selected, and their nitrogenase activities, symbiotic effects on plant growth, and nodule electronmicroscope were compared with those of the wild type. After NTG treatment, as a mutagen, at the concentration exhibiting 99.7% lethal rate, 5 strains of streptomycin resistant mutant having nodulating ability were selected. Among these nodulating mutant strains, 3 strains produced more nodules and 2 strains showed less nodules than wild type. But their nitrogenase activities were decreased significantly, and nodule formation time was also delay compared with those of the wild type, and there was no remarkable difference in effects on plant growth. Microstructure of nodules by electronmicroscopy had mant distinctive differences between red clover nodules inoculated with wild type and mutants.

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