Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Characterization of methionine analogue-resistant mutant of cephalosporium acremonium

메치오닌 유사체 내성 cephalosporium acremonium 변이주의 특성

  • Lee, Kyoung (Genetic Engineering Center, Korea Advanced Institute of Science and Technology) ;
  • Choi, Sang-Ho (Genetic Engineering Center, Korea Advanced Institute of Science and Technology) ;
  • Lee, Jung-Joon (Genetic Engineering Center, Korea Advanced Institute of Science and Technology) ;
  • Mheen, Tae-Ick (Genetic Engineering Center, Korea Advanced Institute of Science and Technology)
  • 이경 (한국과학기술원 유전공학센터 미생물공학연구실) ;
  • 최상호 (한국과학기술원 유전공학센터 미생물공학연구실) ;
  • 이정준 (한국과학기술원 유전공학센터 미생물공학연구실) ;
  • 민태익 (한국과학기술원 유전공학센터 미생물공학연구실)
  • Published : 1987.09.01
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Abstract

Cephalosporium acremonium MAR-80, a strain of methionine analogue-resistant mutant, showed good activity of sulfate utilization as only sulfur source. The effect of methionine on the sulfate uptake system was investigated by using $Na_{2}^{35}SO_{4}$ as a tracer in the resting cell system. From this result, it was revealed that sulfate permease of this strain was less repressed and/or less inhibited by methionine than parent type. This deregulation was due to low actibity of methionine uptake, which was operated by somewhat simple diffusion. From these studies, it could by anticipated that the improved productivity of cephalosporin C and lower dependence of cephalosporin C production on methionine were related to increased uptake rate of sulfate.

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